Abstract: Objective　To establish a mouse model of colorectal cancer liver metastasis (CRLM) by using a modified superior mesenteric vein injection technique, aiming to optimize modeling efficiency and stability, thereby providing a reliable animal model for in vivo experimental research on CRLM.Methods Forty male BALB/c mice were selected. The distal superior mesenteric vein was ligated, and 100 μL of a CT26-luc colorectal adenocarcinoma cell suspension (1×106 cells/mL) was injected to construct the CRLM model. Model establishment was verified using small animal in vivo fluorescence imaging and hematoxylin-eosin (HE) staining.Results　In vivo fluorescence imaging revealed punctate fluorescent signals in the livers of some mice as early as day 2 post-modeling. Stable tumor formation was generally achieved within 4-6 d, with a modeling success rate of 95% and a mortality rate of 5%. Gross anatomical examination showed multiple, scattered grayish-white metastatic foci on the liver surface. HE staining confirmed the presence of multiple, dispersed nuclear atypia lesions within liver tissue. Conclusion　The modified method for establishing mouse CRLM model is highly stable and reproducible, and can serve as a high-quality animal model to support fundamental research on the diagnosis and comprehensive treatment of CRLM.

Key words: mouse model, colorectal cancer liver metastasis, superior mesenteric vein injection

摘要： 目的　通过改良版肠系膜上静脉注射法构建小鼠结直肠癌肝转移(CRLM)模型,优化模型构建效率与稳定 性,为CRLM的相关体内实验研究提供可靠动物模型。方法　选择40只雄性BALB/c小鼠,将小鼠肠系膜上静脉 远端结扎,注入结直肠腺癌细胞CT26-luc悬液100 μL(每mL 1×106个),构建小鼠CRLM模型。通过小动物活体 荧光成像及苏木精-伊红(HE)染色验证模型构建效果。结果　小动物活体荧光图像显示部分小鼠造模第2天可见 点状肝肿瘤荧光影,4~6 d可稳定成瘤,成模率达95%,死亡率为5%。大体解剖可见肝表面多发、散在灰白色转移 病灶。HE染色可见肝组织内多发、散在核异型病灶。结论　改良版小鼠CRLM造模方法稳定性好,成模率高,可 为开展CRLM诊断与综合治疗相关的基础研究提供优质的动物模型支撑。

关键词: 小鼠模型, 结直肠癌肝转移, 肠系膜上静脉注射